HIF-1 alpha Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 13 publications

Catalog Number	Mfr. No.	Quantity
NB100654SS	Novus Biologicals NB100654SS	0.025 mL
NB100654	Novus Biologicals NB100654	0.1 mL

Description

HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Porcine, Bovine samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Gel Super Shift Assays, Knockdown Validated.

Specifications

• Antigen: HIF-1 alpha
• Dilution: Western Blot 1:1000, Simple Western 1:100, Immunohistochemistry 1:100 - 1:300, Immunocytochemistry/Immunofluorescence 1:10 - 1:500. Use reported in scientific literature, Immunohistochemistry-Paraffin 1:100 - 1:300. Use reported in scientific literature, Immunohistochemistry-Frozen 1:100 - 1:300. Use reported in scientific literature, Gel Super Shift Assays, KnockDown Validated reported in scientific literature (PMID 32054096)
• Classification: Polyclonal
• Host Species: Rabbit
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Formulation: PBS with 0.02% Sodium Azide
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Gene Symbols: HIF1A
• Isotype: IgG
• Purification Method: Affinity Purified
• Test Specificity: Human and porcine. Does not react with rat. Immunogen sequence has 93% homology to bovine and 90% homology to mouse.Mouse reactivity reported in scientific literature (PMID: 23959856) Rat reactivity reported in scientific literature (PMID: 24122166).

• Concentration: 1.0 mg/mL
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Gel Shift, KnockDown
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Bovine
• Gene Accession No.: Q16665
• Gene ID (Entrez): 3091
• Immunogen: This HIF-1 alpha Antibody was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
• Primary or Secondary: Primary
• Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 93 kDa

For Research Use Only