Cytochrome c Antibody (7H8.2C12) - BSA Free, Novus Biologicals™

Catalog No.	Quantity
NB10056503T	0.025 mg
NB10056503	0.1 mg

Catalog No. NB10056503T Supplier Novus Biologicals Supplier No. NB10056503SS

Description

Mouse Monoclonal Antibody has been used in 54 publications

Cytochrome c Monoclonal specifically detects Cytochrome c in Human, Mouse, Rat, Drosophila, Rabbit samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoblotting, Flow (Cell Surface), Flow (Intracellular).

Specifications

• Antigen: Cytochrome c
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Classification: Monoclonal
• Clone: 7H8.2C12
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Dilution: Western Blot 0.05-0.5 ug/ml, Simple Western 2.5 ug/ml, Flow Cytometry 1:20-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500. Use reported in scientific literature (Yamasaki et al (2006)), Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (Fujimara et al (1998)), Immunoblotting reported in scientific literature (PMID 16436379), Flow (Cell Surface) reported in scientific literature (PMID 16467206), Flow (Intracellular) reported in scientific literature (Mohr et al (2004))
• Gene Accession No.: P99999
• Gene Alias: CYCHCS, cytochrome c, cytochrome c, somatic, THC4
• Gene Symbols: CYCS
• Host Species: Mouse
• Immunogen: Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
• Purification Method: Protein G purified
• Quantity: 0.025 mg
• Regulatory Status: RUO
• Research Discipline: Apoptosis, Cellular Markers, Core ESC Like Genes, Lipid and Metabolism, Mitochondrial Markers, Stem Cell Markers
• Primary or Secondary: Primary
• Gene ID (Entrez): 54205
• Test Specificity: This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
• Target Species: Human, Mouse, Rat, Drosophila, Rabbit
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Form: Purified
• Isotype: IgG2b κ

For Research Use Only