PCNA Antibody (PC10), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 35 publications

Catalog Number	Mfr. No.	Quantity
NB500106SS	Novus Biologicals NB500106SS	0.025 mL
NB500106	Novus Biologicals NB500106	0.1 mL

Description

PCNA Monoclonal specifically detects PCNA in Human, Mouse, Rat, Porcine, Chicken, Drosophila, Fish, Marsupial, Primate, Rabbit, Yeast, Zebrafish samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating.

Specifications

• Antigen: PCNA
• Concentration: 1 mg/mL
• Applications: Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Autophagy, Base Excision Repair, Cellular Markers, DNA Repair, Loading Controls
• Gene Accession No.: P12004
• Gene ID (Entrez): 5111
• Immunogen: Protein A-rat PCNA fusion obtained from pC2T
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 30 kDa

• Clone: PC10
• Dilution: Western Blot 1:2000, Simple Western 1:200, Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 26883631), Flow Cytometry 1:10-1:1000, ELISA reported in scientific literature, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:1000-1:2000. Use reported in scientific literature (PMID 22068968), Immunoprecipitation 1:100, Immunohistochemistry-Paraffin 1:500-1:1000, Immunohistochemistry-Frozen 1:10-1:500, Immunohistochemistry Free-Floating reported in scientific literature (PMID 27212918), Chromatin Immunoprecipitation (ChIP) 1:10-1:500
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Porcine, Chicken, Drosophila, Fish, Marsupial, Primate, Rabbit, Yeast, Zebrafish
• Gene Alias: cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
• Gene Symbols: PCNA
• Isotype: IgG2a κ
• Purification Method: Protein A purified
• Test Specificity: Specific for PCNA p36 protein expressed at high levels in proliferating cells.

For Research Use Only