PARP Mouse anti-Human, Unlabeled, Clone: 7D3-6, BD

Mouse Monoclonal Antibody

Includes: Purified Mouse Anti-Human PARP, Jurkat Cell Lysate

Catalog Number	Mfr. No.	Quantity
BDB551024	BD Biosciences 551024	50 μg
BDB551025	BD Biosciences 551025	150 μg

Description

PARP [Poly(ADP-ribose) polymerase] is a 116kDa nuclear chromatin-associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in nonapoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular response to DNA damage. Additionally, PARP is a target of the caspase protease activity associated with apoptosis. During apoptosis, PARP is cleaved from the 116kDa intact form into 85kDa and 25kDa fragments. This process separates the amino-terminal DNA-binding domain of the enzyme from the carboxy-terminal catalytic domain resulting in the loss of normal PARP function. Although the role of PARP in apoptosis remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis.

Host Species: Mouse
Clone: 7D3-6
Isotype: IgG1
Species Reactivity: Human
Immunogen: Purified human PARP

Flow Cytometry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: PARP
• Concentration: 0.25mg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Immunogen: Purified human PARP
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

• Clone: 7D3-6
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1
• Purification Method: Affinity Purified

For Research Use Only.