Rab5 Mouse, Unlabeled, Clone: 1, BD

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB610724	BD Biosciences 610724	50 μg
BDB610725	BD Biosciences 610725	150 μg

Description

Rab5 is a low molecular weight GTP-binding protein that plays a role in endocytic vesicle traffic. Like other Rab proteins, Rab5 has C-terminal cysteine residues that are post translationally modified by geranylgeranylation which is critical for its membrane targeting. Rab5 is associated with early endosome and plasma membranes and evidence suggests that Rab5 regulates early endosome fusion. The GTP/GDP cycle controls shuttling of Rab proteins between the cytosol and membranes. In vitro, Rab5 proteins are removed from membranes by a GDP dissociation inhibitor protein (rabGDI) which leads to the formation of a cytosolic Rab5-rabGDI complex. Rab5 may insert into membranes by a multistep process in a which a transient GDP-Rab5 intermediate is formed and converted into GTP-Rab5 that subsequently enters the acceptor membrane and releases rabGDI into the cytosol.

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting

Specifications

• Antigen: Rab5
• Concentration: 250μg/mL
• Classification: Monoclonal
• Host Species: Mouse
• Research Discipline: Cell Biology
• Formulation: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
• Isotype: IgG2a
• Purification Method: Affinity Purified

• Clone: 1
• Applications: Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Canine, Human
• Immunogen: Human Rab5 aa. 1-215
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at -20°C.

For Research Use Only.