Applied Biosystems™ Fast SYBR™ Green Master Mix

Description

Includes

SYBR Green I Dye, AmpliTaq Gold Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference I and optimized buffer components

Requires

Template and primers

Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

• A fast, reliable, and cost-effective solution for real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency
• Real-time PCR results in as fast as 35 minutes
• Capable of detecting very low copies of target
• Minimizes primer-dimer and non-specific amplification
• Consistent amplification across a wide dynamic range
• Contains all of the components, excluding the template and primers, in a convenient 2X master mix
• Store at -5°C to -30°C

Includes the following components in an optimized buffer:

• AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start
  • Minimizes non-specific product formation and enables reactions to be set up at room temperature
• SYBR™ Green I dye to enable detection of double-stranded DNA
• Deoxynucleotides (dNTPs) to help maintain optimal PCR results
• Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
• Passive internal reference based on proprietary ROX™ dye to enable increased precision

Order Info

Shipping Condition: Dry Ice

Specifications

• Concentration: 2X
• Content And Storage: Contains 2 × 5 mL vials. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Sufficient for 1000 reactions of 20 μL each.
  
  Store at -5 to -30°C.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
  
  
• Detection Method: SYBR
• Format: Vial
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
• Reaction Speed: Fast
• For Use With (Equipment): 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
• Label or Dye: SYBR Green
• Passive Reference Dye: ROX (Pre-mixed)
• Product Line: SYBR™
• Product Type: Real Time PCR SYBR Master Mix
• Purity or Quality Grade: UP (Ultra Pure)
• Quantity: 2 x 5 mL
• Sufficient For: 1000 Reactions of 20 μL Each
• For Use With (Application): Gene Expression
• No. of Reactions: 1000 Reactions
• Sample Type: DNA (Genomic), cDNA
• Volume: 2 x 5 mL

For Research Use Only. Not for use in diagnostic procedures.