SYBR™ Green PCR Master Mix Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Everything needed for SYBR Green dye-based PCR amplification and detection in a convenient, single-tube format

Manufacturer: Applied Biosystems 4309155

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Catalog No. 43-091-55

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    Specifications

    For Use With (Application) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
    Includes 2X mixture of SYBR Green I Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components
    Quantity 1 x 5mL

    SYBR Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

    • This mix combines SYBR Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial
    • Premixed components significantly reduce assay setup time
    • SYBR Green I dye detects double-stranded DNA, so specific probes are not required
    • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
    • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
    • Proprietary buffer enhancements ensure performance and reliability

    Maximum Flexibility and Convenience SYBR Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required. SYBR Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR-related fluorescence fluctuations which minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation.

    Gene Expression Analysis & Genotyping, PCR & Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling

    This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

  • Description & Specifications

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