Applied Biosystems™ SYBR™ Select Master Mix for CFX

A cost-effective solution for real-time PCR applications.

Supplier:  Applied Biosystems™ 4472937

Catalog No. 44-729-37

Includes: 2X mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components.

Description

• Specific - minimize primer-dimer and non-specific amplification
• Reproducible and sensitive - consistent amplification across a wide dynamic range
• Bright - contains SYBR™ GreenER™ dye for maximum brightness
• Carry-over contamination control - contains heat-labile UDG
• For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
• Standard and fast cycling parameters

Formulated for Maximum Specificity:

• SYBR™ Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix
• The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:

• SYBR™ Select Master Mix for CFX is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA.
  
  • Contains SYBR™ GreenER™ Dye and Heat-labile UDG:
  • SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye, resulting in brighter signals
  • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

  Order Info

  Shipping Conditions: Wet Ice

Specifications

• Content And Storage: The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100 reactions at 20 μL. Store at 2-8°C.
• Form: Liquid
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq DNA Polymerase
• For Use With (Equipment): AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Real Time PCR SYBR Master Mix
• Quantity: 1 x 1 mL
• Sufficient For: 100 Reactions of 20 μL
• Concentration: 2X
• No. of Reactions: 100 Reactions
• Volume: 1 mL

• Detection Method: SYBR
• Format: Vial
• PCR Method: qPCR
• Reaction Speed: Fast or Standard
• Label or Dye: SYBR GreenER
• Product Line: SYBR™
• Purity or Quality Grade: UP (Ultra Pure)
• Shipping Condition: Wet Ice
• For Use With (Application): Gene Expression
• Hot Start: Built-In Hot Start
• Sample Type: DNA (Genomic), cDNA

For Research Use Only. Not for use in diagnostic procedures.