Applied Biosystems TaqMan PreAmp Master Mix
TaqMan PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.
Manufacturer: Applied Biosystems 4488593
DescriptionTaqMan PreAmp Master Mix lets you: • Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE) • Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan Gene Expression assays • Analyze up to 100 gene expression targets with minimal hands-on time Preserves Equilibrium of Targets Without Preamplification Bias: In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA. Easy Workflow: With TaqMan PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp 9700 thermal cycler. For Research Use Only. Not for use in diagnostic procedures.
Guaranteed minimum shelf life is 60 days.
|Detection Method: Primer-Probe Detection, PCR Method: qPCR|
|Real time PCR (qPCR), real time PCR-based gene expression profiling|
|Contains 2X TaqMan™ PreAmp Master Mix (1mL).|
|Passive Reference Dye: No ROX|
|7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7|
|Store at 2° to 8°C.|
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