TaqMan™ Universal Master Mix II with UNG Non-distribution product as customer accommodation.

Amplify cDNA and DNA targets for quantitation, genotyping and other applications

Manufacturer: Applied Biosystems 4440044

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Catalog No. 44-400-44

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Required Accessories : Primers, probes, template, water

    Specifications

    Detection Method Primer-Probe Detection
    Dye Type ROX (Pre-mixed)
    For Use With (Application) Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real Time PCR-Based miRNA Analysis, miRNA and Non-Coding RNA Analysis
    Quantity 2 x 5mL
    Sample Type DNA (Genomic), cDNA
    Shelf Life Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
    Storage Requirements Store at 4°C

    TAqMan Universal Master Mix II is a convenient mix of components including Uracil-N glycoslyase (UNG) necessary to perform real-time polymerase chain reaction (PCR). Use TAqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for variety of applications, including quantitation and genotyping.

    • Supplied as 2X concentrate
    • Contains AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure), dNTPs (with dUTP), OX™ Passive Reference, Uracil-N glycosylase (UNG) and Optimized buffer components
    • Optimized for use with primers and TAqMan probes
    • Used with custom TAqMan assays available from our custom assay service, or with pre-optimized assays such as: TAqMan Gene Expression Assays, TAqMan MicroRNA Assays, TAqMan Drug Metabolism Genotyping Assays and TAqMan SNP Genotyping Assays
    • For RNA quantitation experiments, it can be used in second step of two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol
    • cDNA template used with master mix can be generated in reverse transcription reaction using cDNA synthesis kits

    Gene Expression Analysis and Genotyping, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling, Real Time PCR-Based miRNA Analysis, miRNA and Non-Coding RNA Analysis

  • Description & Specifications

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