Invitrogen™ Calcium Green™-1, AM, cell permeant

Description

Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺

• They have uses in many calcium signaling investigations, including measuring intracellular Ca²⁺, following Ca²⁺ influx and release, and multiphoton excitation imaging of Ca²⁺ in living tissues
• Cells may be loaded with the AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells
• Fluorescence signal from these cells is generally measured using fluorescence microscopy, fluorescence microplate assays, or flow cytometry
• Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength
• These probes are excited by visible light, and because energy required for excitation is low, potential for cellular photodamage is reduced
• Commonly used laser-based instruments (i.e., confocal laser scanning microscopes) are able to efficiently excite these indicators, and their emissions are in regions of spectrum where cellular autofluorescence and scattering backgrounds are often less of a problem

• Label (Ex/Em): Calcium Green; -1 (506/531nm)
• Fluorescence intensity increase upon binding Ca²⁺ : approximately 14 fold

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• Detection Method: Fluorescence
• For Use With (Application): Cell Viability and Proliferation
• For Use With (Equipment): Fluorescence Microscope
• Product Type: Calcium Indicator
• Dye Type: Fluorescent Dye-Based
• Product Line: Calcium Green
• Quantity: 10 x 50μg
• Shipping Condition: Room Temperature

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.