Description: The TR75-54 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor II (TNFRII, TNFR-p80, TNFRSF1B, CD120b). TNFRII is expressed in a variety of cell types and strongly expressed on stimulated T and B lymphocytes. TNFRII is the main TNF receptor found on circulating T cells and is the major mediator of autoregulatory apoptosis in CD8+ cells. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-alpha and TNF-beta. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release. Applications Reported: This TR75-54 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and ELISA. Applications Tested: The TR75-54 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse TNFRII in combination with the biotin TR75-32 (cat. 13-1204) antibody for detection and recombit mouse TNFRII as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.
|CD120b (TNF Receptor II)|
|ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot|
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
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