Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

CD182 (CXCR2) Mouse anti-Human, Clone: eBio5E8-C7-F10 (5E8-C7-F10), eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$64.50 - $282.00

Specifications

Antigen CD182 (CXCR2)
Clone eBio5E8-C7-F10 (5E8-C7-F10)
Target Species Human
Conjugate Unconjugated
Isotype IgG1, kappa
View More Specs

Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-129-50 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14182980
25 μg Each for $64.50
Add to cart
 
50-129-52 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14182985
500 μg Each for $282.00
Add to cart
 
50-129-51 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14182982
100 μg Each for $117.00
Add to cart
 
Description

Description

Description: The eBio5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8Rbeta). CD182 is a 67-70 kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8Ralpha) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it ft.s ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, -beta and -gamma, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predomitly mediates its effects on neutrophil function through CD181. Applications Reported: This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. CXCR2 is a receptor for interleukin-8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to GRO/MGSA and NAP-2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.
Specifications

Specifications

CD182 (CXCR2)
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C
eBio5E8-C7-F10 (5E8-C7-F10)
Unconjugated
Flow Cytometry
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit