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Promega DNA-Dependent Protein Kinase

Catalog No. PRZV5811 Shop All Promega Corporation Products

Description

Phosphorylates several DNA-binding substrates in vitro, including the tumor suppressor protein p53, the SV40 large T-antigen and several transcription factors

  • Isolated from HeLa nuclear extracts as a complex consisting of a 400kDa catalytic subunit and a 155kDa heterodimeric DNA-binding component named Ku, which itself consists of subunits of approximately 85kDa and 70kDa
  • Unit Definition: One unit is defined as the amount of enzyme required to transfer 1pmol of phosphate to DNA-Dependent Protein Kinase Peptide Substrate in 1 minute at 30°C
  • Storage Buffer: 25mM HEPES (pH 7.5), 50mM KCl, 0.2mM EDTA, 10mM MgCl2, 1mM DTT, 10% glycerol

Specifications

Product Type Protein Kinase
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