Enolase 2/Neuron-specific Enolase Mouse anti-Human, Clone: ENO2/1462, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP25960220	Novus Biologicals NBP25960220UG	20 μg
NBP259602X	Novus Biologicals NBP259602100UG	100 μg

Description

Enolase 2/Neuron-specific Enolase Monoclonal specifically detects Enolase 2/Neuron-specific Enolase in Human samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array.

Specifications

• Antigen: Enolase 2/Neuron-specific Enolase
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), SDS-Page
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cellular Markers, Neuronal Cell Markers, Neuroscience
• Formulation: 10mM PBS with 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 2026
• Immunogen: A synthetic peptide of human NSE gamma (around aa416-433; exact sequence is proprietary)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 50 kDa

• Clone: ENO2/1462
• Dilution: Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 1-2 ug/ml, Immunohistochemistry-Paraffin 0.1-0.2 ug/ml, SDS-Page, Protein Array 1:100-1:2000
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: 2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, Enolase 2, enolase 2 (gamma, neuronal), gamma-enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuron-specific enolase, NSE
• Gene Symbols: ENO2
• Isotype: IgG2b
• Purification Method: Protein A or G purified
• Test Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of about 50kDa, which is identified as gamma -enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

For Research Use Only