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Invitrogen™ EnzChek™ Phosphatase Assay Kit
Description
Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes (proprietary) 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate. DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ∽1000 assays, using a reaction volume of 100μL per assay. Fluorescence (excitation/emission maxima approx. 358/455nm) can be measured in a fluorescence microplate reader or a standard fluorometer.
Cell Analysis, Cellular Imaging, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Color | Blue |
| Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Application) | Phosphatase Assay |
| For Use With (Equipment) | Microplate Reader |
| Product Type | Phosphatase Assay |
| Dye Type | DiFMU |
| Emission | 360/460 |
| Product Line | EnzChek |
| Quantity | 1000 Assays |
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For Research Use Only. Not for use in diagnostic procedures.
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