Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Description

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.

• Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity - detects low copy number targets
• Wide linear range - accurate quantification across 9 orders of magnitude
• Reproducibility and convenience - ready-to-use 2X master mix

Specifications

• Concentration: 2X
• Content And Storage: Contains:
  • 2 x 1.25 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 200 x 25 μL reactions.
  • 50 μL ROX solution, 50 μM
  • 2 x 1.25 mL Nuclease-Free Water
  
  Store at -20°C.
• Detection Method: SYBR
• Format: Tube
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: Taq DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
• Product Line: Maxima
• Product Type: Real Time PCR SYBR Master Mix
• Quantity: 200 reactions
• Sufficient For: 200 Reactions of 25 μL
• For Use With (Application): Gene Expression
• No. of Reactions: 200 reactions
• Sample Type: DNA, dsDNA
• Volume: 2 x 1.25 mL

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.