Thermo Scientific™ Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Supplier:  Thermo Scientific™ K0253

Catalog No. FERK0253

Description

The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.

• Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity - detects low copy number targets
• Wide linear range - accurate quantification across 9 orders of magnitude
• Reproducibility and convenience - ready-to-use 2X master mix

Specifications

• Content And Storage: Contains:
  • 4 x 12.5 mL Maxima SYBR Green qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 4000 x 25 μL reactions.
  • 1 mL ROX solution, 50 μM
  • 2 x 30 mL Nuclease-Free Water
  
  Store at -20°C.
• Format: Tube
• PCR Method: qPCR
• Reaction Speed: Standard
• Product Line: Maxima
• Sufficient For: 4000 Reactions of 25 μL
• Concentration: 2X
• Sample Type: DNA, dsDNA

• Detection Method: SYBR
• GC-Rich PCR Performance: High
• Polymerase: Taq DNA Polymerase
• For Use With (Equipment): BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
• Product Type: Real Time PCR SYBR Master Mix
• For Use With (Application): Gene Expression
• No. of Reactions: 4,000 Reactions
• Volume: 4 x 12.5 mL

For Research Use Only. Not for use in diagnostic procedures.