GE Healthcare Con A Sepharose™ 4B Medium Available on GSA/VA Contract for Federal Government customers only.

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

Manufacturer: GE Healthcare 17044001

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Catalog No. 45-000-068

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    Specifications

    Binding Capacity 30mg/mL
    For Use With (Application) For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes
    Ligand Concanavalin A
    Ligand Concentration 13mg Con A/mL Drained Medium
    Matrix 4% Agarose
    Particle Size 90μm
    pH Stability 4 to 9
    Quantity 100mL
    Storage Requirements 2° to 8°C

    Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.

    • Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
    • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
    • Group-specific adsorbent for molecules containing sugars
    • Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
    • Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
    • Average particle size: 90µm
    • Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl2, 1mM MgCl2, 1mM MnCl2, and 20% ethanol

  • Description & Specifications

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