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GE Healthcare Lentil Lectin Sepharose™ 4B Medium

Routinely used in the preparation and purification of a number of glycoproteins and carbohydrate-containing molecules

Manufacturer:  GE Healthcare 17044401

Catalog No. 45-000-070


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Description

Description

  • Lentil lectin coupled to Sepharose 4B via cyanogen bromide activation
  • Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars
  • Retains binding in 1% deoxycholate; especially suited for purification of detergent-solubilized membrane proteins
  • Ligand concentration 2 mg Lentil lectin/ml; binding capacity for porcine thyroglobulin approx. 16mg/mL medium
  • Average particle size 90µm
  • Stable in the pH range 3-10, and in the presence of all commonly used buffers (Chelating agents and 8M urea to be avoided)
  • Has more equal affinity for α-D-mannose and α-D-glucose compared to Con A

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Supplied in 20% ethanol containing 0.9% NaCl, 1mM Ca2+ and 1mM Mn2+

Specifications

Specifications

25mg/mL
Lentil lectin
4% Agarose
3 to 10
2° to 8°C
Used for purification of a number of Glycoproteins and other Carbohydrate containing molecules
2mg Lentil lectin/mL Medium
90μm
25mL
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