GE Healthcare Protein A and Protein G Mag Sepharose™ Available on GSA/VA Contract for Federal Government customers only.

Manufacturer: GE Healthcare 28944008

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Catalog No. 45-002-160

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    Specifications

    Bead Type Protein G Mag Sepharose
    Binding Capacity 13 to 22mg Human IgG/mL
    For Use With (Application) Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation applications
    Ligand Protein G
    Matrix Paramagnetic spherical, highly cross linked agarose particles
    Particle Size 37 to 100μm
    Quantity 500μL
    Storage Requirements 4° to 8°C

    Protein A and Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A or native protein G as ligand. Protein A and Protein G have high affinities for the Fc region of IgG antibodies and offer differing affinity characteristics across a broad range of mammalian IgG species.

    There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for for downstream applications such as electrophoresis and MS.

    Protein A and Protein G Mag Sepharose combine well-proven enrichment methods with the magnetic beads platform, which has ideal properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

    • Visible and dense Sepharose based magnetic beads with protein A or protein G ligand, easy to spot and collect bound target protein
    • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
    • Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
    • Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed

    Matrix: Paramagnetic, spherical, highly cross-linked agarose particles
    Ligand: Native protein A; Native protein G
    Binding capacity Protein A: 8-17mg human IgG/mL gel
    Binding capacity Protein G: 13-22mg human IgG/mL gel
    Particle size: 37-100µm
    Working temperature: Room temperature
    Storage solution: 20% ethanol
    Storage temperature: 4°C to 8°C

  • Description & Specifications

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