GE Healthcare Sepharose™ Fast Flow Cation Exchange Media Available on GSA/VA Contract for Federal Government customers only.

Used for preparative protein separations in both research and industrial applications. GE Healthcare CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

Manufacturer: GE Healthcare 17071901

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Catalog No. 45-002-931

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    Anion Exchange Capacity 0.09 to 0.13mM Hl+/mL
    Anion Exchange Type Weak CatIon Exchanger
    Binding Capacity 50mg Ribonuclease A/mL medium
    Flow Rate 300 to 600cm/Hour
    For Use With (Application) For preparative Protein separations in research and industrial appliCatIons, for capture and intermediate purifiCatIon of wide range of approved biopharmaceuticals
    Ligand Type Carboxymethyl
    Matrix 6% Cross-Linked Agarose
    Max. Pressure 100kPa
    Packaging Quantity 500mL
    Quantity 500mL
    Stability Stable in commonly used aqueous Buffers: 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
    Storage Buffer 20% Ethanol
    Storage Requirements 4 to 30°C
    Particle Size 45 to 165μm
    Product Type CM Sepharose fast flow media
    pH Range 4 to 13

    • Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
    • Composed of crosslinked 6% agarose beads
    • Reproducible
    • Part of Sepharose Fast Flow ion exchange platform
    • High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
    • Available in range of pre-packed process development tools

    SP Sepharose:

    • Ligand: Sulphopropyl
    • Ion Exchanger Type: Strong cation exchanger
    • Ionic Capacity: 0.18 to 0.25mmol H+/mL medium
    • Binding capacity: 70mg ribonuclease A/mL medium
    CM Sepharose:
    • Ligand: Carboxymethyl
    • Ion Exchanger Type: Weak cation exchanger
    • Ionic Capacity: 0.09 to 0.13mmol Hl+/mL medium
    • Binding capacity: 50mg ribonuclease A/mL medium

    Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

  • Description & Specifications

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