HIF-1 alpha Antibody (H1alpha67), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 891 publication

Catalog Number	Mfr. No.	Quantity
NB100105SS	Novus Biologicals NB100105SS	0.025 mL
NB100105	Novus Biologicals NB100105	0.1 mL

Description

HIF-1 alpha Monoclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Porcine, Bovine, Canine, Feline, Ferret, Monkey, Primate, Rabbit, Sheep, Xenopus samples. It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoassay, Immunoblotting, In vitro assay, Gel Super Shift Assays, Proximity Ligation Assay, Tissue Culture Substratum, Ligand Activation, Immunohistochemistry Free-Floating, Knockout Validated, Knockdown Validated.

Specifications

• Antigen: HIF-1 alpha
• Concentration: 1.0 mg/mL
• Applications: Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA, In vitro Assay, KnockDown
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Gene Accession No.: Q16665
• Gene ID (Entrez): 3091
• Immunogen: This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 93 kDa

• Clone: H1alpha67
• Dilution: Western Blot 1:500, Simple Western, Chromatin Immunoprecipitation 1 - 5 ug/IP. Use reported in scientific literature, Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000. Use reported in scientific literature (PMID 20042684), Immunohistochemistry 1:20-1:50, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:20-1:50, Immunohistochemistry-Frozen 1:20-1:50, Immunoassay reported in scientific literature (PMID 26147748), Immunoblotting reported in multiple pieces of scientific literature, In vitro assay reported in multiple pieces of scientific literature, Gel Super Shift Assays 1:1 - 1:100. Use reported in scientific literature (PMID 22411794), Proximity Ligation Assay reported in scientific literature (PMID 27595394), Tissue Culture Substratum, Ligand Activation reported in scientific literature (PMID 26147748), Immunohistochemistry Free-Floating reported in scientific literature (PMID 33242463), Chromatin Immunoprecipitation (ChIP) 1-5 ug/IP, Knockout Validated reported in scientific literature (PMID 26861754), KnockDown Validated reported in scientific literature (PMID 32772041)
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Bovine, Canine, Feline, Ferret, Monkey, Primate, Rabbit, Sheep, Xenopus species
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, H1alpha67, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1
• Gene Symbols: HIF1A
• Isotype: IgG2b
• Purification Method: Protein G purified
• Test Specificity: Reactivity with Candida albicans reported by a customer review. Reactivity with Xenopus reported in the scientific literature (PMID: 18303027).

For Research Use Only