HIF-2 alpha/EPAS1 Antibody (ep190b), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 177 publications

Catalog Number	Mfr. No.	Quantity
NB100132SS	Novus Biologicals NB100132SS	0.025 mL
NB100132	Novus Biologicals NB100132	0.1 mL

Description

HIF-2 alpha/EPAS1 Monoclonal specifically detects HIF-2 alpha/EPAS1 in Human, Mouse, Rat, Bovine, Hamster samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, In vivo assay, Gel Supershift Assay, Knockdown Validated.

Specifications

• Antigen: HIF-2 alpha/EPAS1
• Concentration: 1.0 mg/mL
• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In vitro Assay, KnockDown
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Angiogenesis, Apoptosis, Cancer, Cancer Stem Cells, Chromatin Research, Embryonic Stem Cell Markers, HIF Target Genes, Hypoxia, Lipid and Metabolism, Transcription Factors and Regulators
• Gene Accession No.: Q99814
• Gene ID (Entrez): 2034
• Immunogen: The immunogen recognized by this HIF-2 alpha/EPAS1 Antibody (ep190b) maps to a region between amino acids 535-631. [UniProt# Q99814]
• Primary or Secondary: Primary
• Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 96.5 kDa

• Clone: ep190b
• Dilution: Western Blot 1 - 2 ug/mL, Simple Western 1:100, Flow Cytometry 1:400, ELISA 1:100-1:2000, Immunohistochemistry 1:150 - 1:300, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1:150 - 1:300, Immunohistochemistry-Frozen reported in scientific literature (PMID 24973414), Gel Super Shift Assays reported in scientific literature (PMID 17404621), In vivo assay reported in scientific literature (PMID 23857308), Gel Supershift Assay, Chromatin Immunoprecipitation (ChIP), KnockDown Validated reported in scientific literature (PMID 32054096)
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Bovine, Hamster
• Gene Alias: Basic-helix-loop-helix-PAS protein MOP2, BHLHE73, Class E basic helix-loop-helix protein 73, ECYT4, endothelial PAS domain protein 1, endothelial PAS domain-containing protein 1, ep190b, EPAS1, EPAS-1, HIF-1-alpha-like factor, HIF-1alpha-like factor, HIF-
• Gene Symbols: EPAS1
• Isotype: IgG1
• Purification Method: Protein G purified
• Test Specificity: This HIF-2 alpha/EPAS1 Antibody (ep190b) is specific for HIF-2 alpha/EPAS1, and does not cross-react with HIF-1 alpha.

For Research Use Only