HSP27 Antibody (HSPB1/774), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2449750.2MG

Catalog No. NBP24497502

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

HSP27 Monoclonal antibody specifically detects HSP27 in Human, Mouse, Rat, Chicken, Primate, Sheep samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry-Paraffin, SDS-Page, Immunofluorescence.

Specifications

• Antigen: HSP27
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.25 - 0.5 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P04792
• Gene Symbols: HSPB1
• Immunogen: Recombinant human HSPB1 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
• Clone: HSPB1/774
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: 28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
• Host Species: Mouse
• Molecular Weight of Antigen: 27 kDa
• Quantity: 0.2 mg
• Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience
• Gene ID (Entrez): 3315
• Target Species: Human, Mouse, Rat, Chicken, Primate, Sheep
• Form: Purified

For Research Use Only