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HSP27 Antibody (HSPB1/774), Novus Biologicals™
SDP

Catalog No. NBP24497502 Shop All R&D Systems Products
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NBP24497502 0.2 mg
NBP24497500 0.02 mg
NBP24497501 0.1 mg
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Catalog No. NBP24497502 Supplier Novus Biologicals Supplier No. NBP2449750.2MG

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

HSP27 Monoclonal antibody specifically detects HSP27 in Human, Mouse, Rat, Chicken, Primate, Sheep samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry-Paraffin, SDS-Page, Immunofluorescence.

Specifications

Antigen HSP27
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Classification Monoclonal
Clone HSPB1/774
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.25 - 0.5 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P04792
Gene Alias 28 kDa heat shock protein, DKFZp586P1322, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, HMN2B, HS.76067, Hsp25, HSP27HSP 27, HSP28CMT2F, HspB1, SRP27, Stress-responsive protein 27
Gene Symbols HSPB1
Host Species Mouse
Immunogen Recombinant human HSPB1 protein
Molecular Weight of Antigen 27 kDa
Purification Method Protein A or G purified
Quantity 0.2 mg
Regulatory Status RUO
Research Discipline Cancer, Cell Biology, Cell Cycle and Replication, Golgi Apparatus Markers, Membrane Trafficking and Chaperones, Neuroscience
Primary or Secondary Primary
Gene ID (Entrez) 3315
Test Specificity It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Target Species Human, Mouse, Rat, Chicken, Primate, Sheep
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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For Research Use Only

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