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Invitrogen™ AccuPrime™ Pfx DNA Polymerase
Provides higher fidelity than Pfu DNA polymerase
$509.65 - $2295.65
Specifications
| Detection Method | Primer-probe |
|---|---|
| Format | Tube |
| GC-Rich PCR Performance | Low |
| Polymerase | AccuPrime Pfx DNA Polymerase |
| Reaction Speed | Standard |
Includes: AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity & Availability | |||||
12-344-024
|
Invitrogen™
12344024 |
200 Reactions |
Each
for $509.65
Save 5% Reg : |
|
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|
12-344-032
|
Invitrogen™
12344032 |
1000 Reactions |
Each
for $2,295.65
Save 5% Reg : |
|
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Description
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.
- Achieve greater than 26 times higher fidelity than Taq DNA polymerase
- Amplify fragments up to 12 kb
- Minimize PCR optimization
- Built-in hot start
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
| Primer-probe | |
| Low | |
| Standard | |
| AccuPrime™ | |
| 26 X | |
| Blunt | |
| 12 kb or less |
| Tube | |
| AccuPrime Pfx DNA Polymerase | |
| 3' - 5' | |
| Approved for shipment on Wet or Dry Ice | |
| Built-In Hot Start | |
| Separate Components | |
| DNA |
For Research Use Only. Not for use in diagnostic procedures.
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