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Invitrogen™ AccuPrime™ Pfx DNA Polymerase 
Provides higher fidelity than Pfu DNA polymerase
$408.00 - $1830.00
Specifications
| Fidelity (vs. Taq) | Fidelity (vs. Taq): 26X |
|---|---|
| Gel Compatibility | Gel Compatibility: Low |
| Storage Requirements | Store at -20°C. |
Includes: AccuPrime Pfx DNA Polymerase (100µL, at 2.5U/µL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50µL each.
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
|
12-344-024
|
Invitrogen™
12344024 |
200 reactions |
Each for $408.00
|
|
12-344-032
|
Invitrogen™
12344032 |
1000 reactions |
Each for $1,830.00
|
|
Description
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.
- Achieve greater than 26 times higher fidelity than Taq DNA polymerase
- Amplify fragments up to 12 kb
- Minimize PCR optimization
- Built-in hot start
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research
Order Info
Approved for shipment on Wet or Dry Ice
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Specifications
| Fidelity (vs. Taq): 26X | |
| Gel Compatibility: Low | |
| Store at -20°C. |
For Research Use Only. Not for use in diagnostic procedures.
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