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Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase

$509.65 - $2295.65

Specifications

Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance Low
Polymerase AccuPrime Pfx DNA Polymerase
Reaction Speed Standard
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Includes: AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.

Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
12-344-024
Encompass_Preferred
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Invitrogen™
12344024
200 Reactions
Each for $509.65

Save 5%

Reg : $536.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
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12-344-032
Encompass_Preferred
View Documents
Invitrogen™
12344032
1000 Reactions
Each for $2,295.65

Save 5%

Reg : $2,410.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
Learn More
Add to Cart
 
Description

Description

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.

  • Achieve greater than 26 times higher fidelity than Taq DNA polymerase
  • Amplify fragments up to 12 kb
  • Minimize PCR optimization
  • Built-in hot start

Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Specifications

Primer-probe
Low
Standard
AccuPrime™
26 X
Blunt
12 kb or less
Tube
AccuPrime Pfx DNA Polymerase
3' - 5'
Approved for shipment on Wet or Dry Ice
Built-In Hot Start
Separate Components
DNA
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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