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Invitrogen™ AccuPrime™ Pfx DNA Polymerase
Provides higher fidelity than Pfu DNA polymerase
$509.65 - $2295.65
Specifications
Detection Method | Primer-probe |
---|---|
Format | Tube |
GC-Rich PCR Performance | Low |
Polymerase | AccuPrime Pfx DNA Polymerase |
Reaction Speed | Standard |
Includes: AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.
Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity & Availability | |||||
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Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity & Availability | |||||
12-344-024
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Invitrogen™
12344024 |
200 Reactions |
Each
for $509.65
Save 5% Reg : |
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12-344-032
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Invitrogen™
12344032 |
1000 Reactions |
Each
for $2,295.65
Save 5% Reg : |
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Description
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.
- Achieve greater than 26 times higher fidelity than Taq DNA polymerase
- Amplify fragments up to 12 kb
- Minimize PCR optimization
- Built-in hot start
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
Primer-probe | |
Low | |
Standard | |
AccuPrime™ | |
26 X | |
Blunt | |
12 kb or less |
Tube | |
AccuPrime Pfx DNA Polymerase | |
3' - 5' | |
Approved for shipment on Wet or Dry Ice | |
Built-In Hot Start | |
Separate Components | |
DNA |
For Research Use Only. Not for use in diagnostic procedures.
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