Invitrogen SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Manufacturer: Invitrogen 11736051
- SuperScript III RT for higher temperature cDNA synthesis for greater success with difficult RNA secondary structure
- Platinum Taq DNA Polymerase with hot-start technology for improved specificity
- LUX Fluorogenic Primers (Figure 1) or dual-labeled fluorogenic probes for superior detection performance
- SYBR Green I dye for easy and convenient detection
- Validated performance on multiple real-time instrument platforms, including rotor-based systems
Shipping Conditions: Dry Ice
|7500 System, BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5, Cepheid SmartCycler, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000|
|Store all components at -20°C (-80°C for long-term storage). ROX reference dye must be stored in the dark.|
|Real time PCR (qPCR), real time PCR-based gene expression profiling|
|100μL SuperScript™ III RT/Platinum™ Taq Mix; 2 x 1.25mL 2X SYBR™ Green Reaction Mix; 1mL Magnesium Sulfate (50mM); 100μL ROX Reference Dye (25μM); 300μL 20X Bovine Serum Albumin (1mg/mL)|
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