- Stable—dyes are freeze dried in separate vials to maintain stability
- Robust—staining pattern is the same before and after fixation
- Low compensation—minimal spectral overlap between other fluorophores
- Packaged in 40-test vials to help ensure the stability and performance of the dye over time.
- Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated.
- An amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.
- Fluorescent properties to minimize compensation between other violet dyes and dyes that excite off of the 488 nm blue laser.
- The yellow-fluorescent reactive dye has an excitation maximum of ∼405 nm, but it is excited well with the 405 nm violet laser, and it has an emission maxima of ∼570 nm, so it can be collected in the second or third channel on most violet laser flow cytometers.
- In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining.
- In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence.
- The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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