Melan-A/MART-1 Mouse, Clone: M2-7C10 + M2-9E3, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP234245F	Novus Biologicals NBP2342450.02MG	0.02 mg
NBP234245A	Novus Biologicals NBP2342450.1MG	0.1 mg
NBP234245B	Novus Biologicals NBP2342450.2MG	0.2 mg

Description

Melan-A/MART-1 Monoclonal specifically detects Melan-A/MART-1 in Human, Mouse, Rat samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

• Antigen: Melan-A/MART-1
• Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat
• Gene Accession No.: Q16655
• Gene ID (Entrez): 2315
• Immunogen: Recombinant hMART-1 protein (M2-7C10; M2-9E3)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 21 kDa

• Clone: M2-7C10 + M2-9E3
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cytoskeleton Markers, Immunology
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: Antigen LB39-AA, Antigen SK29-AA, Mart 1 Melan A, MART1MART-1, melan-A, melanoma antigen recognized by T-cells 1, Protein Melan-A
• Gene Symbols: MLANA
• Isotype: IgG
• Purification Method: Protein A purified
• Test Specificity: This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.