Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Molecular Probes™ Amplex™ Red Glucose/Glucose Oxidase Assay Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Format allows for multiple time point measurements

Manufacturer:  Invitrogen™ A22189

Catalog No. A22189


Add to cart

Description

Description

The Amplex Red Glucose/Glucose Oxidase Assay Kit provides a sensitive and simple method for detecting d-glucose or glucose oxidase using a fluorescence microplate reader or fluorometer. The Amplex Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) is a colorless, stable, and extremely versatile peroxidase substrate. Because peroxidase- and glucose oxidase-mediated reactions can be coupled, it is possible to measure glucose oxidase activity or the release of glucose by any glucosidase enzyme—for instance, β-glucosidase and glucocerebrosidase—in either a continuous or discontinuous assay. Because of the ability to couple reactions, this assay has been demonstrated to be useful for the quantification of glucose levels in foods, fermentation media, and bodily fluids. In the assay, glucose oxidase reacts with d-glucose to form d-gluconolactone and hydrogen peroxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide then reacts with the Amplex Red reagent in a 1:1 stoichiometric ratio to generate the red-fluorescent product resorufin. Because resorufin has absorption and fluorescence emission maxima of approximately 571nm and 585nm, respectively, there is little interference from autofluorescence in most biological samples.

  • Detect concentrations as low as 3µM d-glucose or 0.05mU/mL of glucose oxidase
  • Format allows for multiple time point measurements
  • Designed for minimal autofluorescence interference
  • Custom assay design and packaging are also available

Use Amplex Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex UltraRed Reagent, a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex Red/UltraRed Stop Reagent. The Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours.

Enzyme and Protein Activity Assays, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Order Info

Shipping Condition: Room temperature

Specifications

Specifications

500 assays
Fluorescent
Kit, Assay
96-well plate, Tube(s)
Store in freezer (-5° to -30°C) and protect from light.
Fluorometer, Microplate Reader, Spectrophotometer
500 assays
Amplex Red
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.