Invitrogen™ Rhod-2, AM, cell permeant

Description

Cells may be loaded with AM ester forms of these calcium indicators by adding dissolved indicator directly to dishes containing cultured cells

Labeled calcium indicators are molecules that exhibit increase in fluorescence upon binding Ca²⁺. They have uses in many calcium signaling investigations, including measuring Ca²⁺ in cells and tissues that have high levels of autofluorescence and also for detecting Ca²⁺ release generated by photoreceptors and photoactivatable chelators.

• Fluorescence signal from these cells is generally measured using fluorescence microscopy
• Label (Ex/Em of Ca²⁺-bound form): Rhod-2 (552/581nm)
• Fluorescence intensity increase upon binding Ca²⁺: >100 fold
• K_d for Ca²⁺ in the absence of Mg²⁺, in buffer: approximately 570nM
• Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelength
• In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn²⁺, Zn²⁺, Pb²⁺) with substantially higher affinity than Ca²⁺
• Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN

Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling

Order Info

Shipping Condition: Room temperature

Specifications

• Content And Storage: Store in freezer -5°C to -30°C and protect from light.
• Detection Method: Fluorescence
• For Use With (Application): Cell Viability and Proliferation
• For Use With (Equipment): Fluorescence Microscope
• Product Type: Stain
• Dye Type: Fluorescent Dye-Based
• Quantity: 1mg
• Shipping Condition: Room Temperature

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.