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Mouse anti-p21/CIP1/CDKN1A, Clone: WA-1 (HJ21), Azide Free, Novus Biologicals™
SDP

Catalog No. NBP23308402 Shop All R&D Systems Products
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0.2 mg
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NBP23308402 0.2 mg
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Catalog No. NBP23308402 Supplier Novus Biologicals Supplier No. NBP2330840.2MG

Specifications

Antigen p21/CIP1/CDKN1A
Applications Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Classification Monoclonal
Clone WA-1 (HJ21)
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunohistochemistry 0.5-1ug/ml, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
Formulation 10mM PBS - BSA free with No Preservative
Gene Alias CAP20cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, CDKN1melanoma differentiation associated protein 6, CIP1WAF1CDK-interaction protein 1, cyclin-dependent kinase inhibitor 1A (p21, Cip1), MDA6, MDA-6, Melanoma differentiation-associated protein 6, p21, p21CIP1, p21Cip1/Waf1, PIC1, SDI1DNA synthesis inhibitor, wild-type p53-activated fragment 1
Gene Symbols CDKN1A
Host Species Mouse
Immunogen Recombinant human p21 protein was used as immunogen to generate the antibody.
Molecular Weight of Antigen 21 kDa
Purification Method Protein G purified
Quantity 0.2 mg
Regulatory Status RUO
Research Discipline Apoptosis, Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific
Primary or Secondary Primary
Gene ID (Entrez) 1026
Test Specificity This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein.
Target Species Human, Mouse, Rat, Primate
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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