Mouse anti-p21/CIP1/CDKN1A, Clone: WA-1 (HJ21), Azide Free, Novus Biologicals™

Supplier:  Novus Biologicals NBP2330840.2MG

Catalog No. NBP23308402

Specifications

• Antigen: p21/CIP1/CDKN1A
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunohistochemistry 0.5-1ug/ml, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, CyTOF-ready
• Gene Alias: CAP20cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, CDKN1melanoma differentiation associated protein 6, CIP1WAF1CDK-interaction protein 1, cyclin-dependent kinase inhibitor 1A (p21, Cip1), MDA6, MDA-6, Melanoma differentiation-associated protein 6, p21, p21CIP1, p21Cip1/Waf1, PIC1, SDI1DNA synthesis inhibitor, wild-type p53-activated fragment 1
• Host Species: Mouse
• Molecular Weight of Antigen: 21 kDa
• Quantity: 0.2 mg
• Research Discipline: Apoptosis, Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific
• Gene ID (Entrez): 1026
• Target Species: Human, Mouse, Rat, Primate
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Clone: WA-1 (HJ21)
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Symbols: CDKN1A
• Immunogen: Recombinant human p21 protein was used as immunogen to generate the antibody.
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only