Notch-1 Antibody - (Cleaved N terminal), Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 8 publications

Supplier:  Novus Biologicals NB300251

Catalog No. NB300251

Description

Notch-1 Polyclonal specifically detects Notch-1 in Human, Mouse samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Notch-1
• Classification: Polyclonal
• Dilution: Western Blot 1:500-1:2000, ELISA 1:20000-1:60000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin 1:200
• Gene Accession No.: P46531
• Gene Symbols: NOTCH1
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch-1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD). (Uniprot: P46531)
• Purification Method: Unpurified
• Regulatory Status: RUO
• Gene ID (Entrez): 4851
• Target Species: Human, Mouse, Rat
• Form: Antisera

• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.1% Sodium Azide
• Gene Alias: EC 2.1.2.11, EC 3.4.21.68, hN1, Notch (Drosophila) homolog 1 (translocation-associated), notch 1Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1
• Host Species: Rabbit
• Molecular Weight of Antigen: 272.5 kDa
• Quantity: 0.2 mL
• Primary or Secondary: Primary
• Test Specificity: This antiserum is directed against human Notch-1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat Notch-1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

For Research Use Only