Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media

Used for purification of monoclonal and polyclonal antibodies. Cytiva Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.

Catalog Number	Mfr. No.	Quantity	pH Range
45-002-981	Cytiva 17528001	5 mL	3 to 9

Description

• Low leakage of protein A with nearly twice total IgG binding capacity of Protein A Sepharose CL-4B
• Used in large-scale FDA-approved processes
• Manufactured without using animal-derived components
• Rigidity and high degree of substitution of Sepharose 4 Fast Flow matrix facilitates speedy process of large volumes dilute cell culture fluid
• High chemical, thermal and mechanical stability
• Withstands high concentrations of hydrogen bond disrupting agents

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Specifications

• Stability: No significant change in chromatographic performance after 1 week storage using 8 M Urea, 6 M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol
• Binding Capacity: >35 mg Human IgG/mL
• Flow Rate: 150 to 250 cm/Hoμr
• Height (English) Bed: 9.84 in.
• Max. Pressure: 100 kPa
• Storage Buffer: 20% Ethanol

• Storage Requirements: 2°C to 8°C
• Product Type: nProtein A Sepharose 4 Fast Flow Media
• Particle Size: 90 μm
• Height (Metric) Bed: 25 cm
• Matrix: 4% Cross-Linked Agarose
• Ligand Type: Native Protein A (S. aureus)