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  6. p21/CIP1/CDKN1A Antibody (DCS-60.2), Novus Biologicals™

p21/CIP1/CDKN1A Antibody (DCS-60.2), Novus Biologicals™
SDP

Catalog No. p-5540043 Shop All R&D Systems Products
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NBP24452500 0.02 mg
NBP24452501 0.1 mg
NBP24452502 0.2 mg
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Catalog No. NBP24452500 Supplier Novus Biologicals Supplier No. NBP2445250.02MG
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Description

Mouse Monoclonal Antibody has been used in 3 publications

Ensure accurate, reproducible results in Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

p21/CIP1/CDKN1A Monoclonal specifically detects p21/CIP1/CDKN1A in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

Antigen p21/CIP1/CDKN1A
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Classification Monoclonal
Clone DCS-60.2
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 1 - 2 ug/ml, Simple Western 10 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 2 - 4 ug/ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P38936, P38936
Gene Alias CAP20cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, CDKN1melanoma differentiation associated protein 6, CIP1WAF1CDK-interaction protein 1, cyclin-dependent kinase inhibitor 1A (p21, Cip1), MDA6, MDA-6, Melanoma differentiation-associated protein 6, p21, p21CIP1, p21Cip1/Waf1, PIC1, SDI1DNA synthesis inhibitor, wild-type p53-activated fragment 1
Gene Symbols CDKN1A
Host Species Mouse
Immunogen Human recombinant p21 protein
Molecular Weight of Antigen 21 kDa
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Apoptosis, Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair
Primary or Secondary Primary
Gene ID (Entrez) 1026
Test Specificity This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Target Species Human, Mouse (Negative), Rat (Negative)
Content And Storage Store at 4C.
Form Purified
Isotype IgG2a κ
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