PEG, Mouse anti-All, Clone: AGP4, Millipore Sigma™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABS196425	MilliporeSigma MABS196425UG	25 μg
MABS1964100	MilliporeSigma MABS1964100UG	100 μg

Description

Anti-PEG, clone AGP4, Cat. No. MABS1964, is a mouse monoclonal antibody that detects Polyethylene glycol (PEG) and has been tested for use in ELISA, Western Blotting, and Immunohistochemistry.

Polyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solution. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. PEG is considered as a non-immunogenic moiety and has been widely tested for its ability to protect proteins from the host s immune system. This antibody (Clone AGP4) binds to the PEG backbone and is suitable for capture of PEGylated compounds in ELISA as well as the detection of PEGylated molecules. It is suitable for sensitive ELISA applications where AGP4 can be used as a capture antibody. Note: Tween-20 should be used in wash buffers for this antibody combination.

Specifications

• Antigen: PEG
• Dilution: Immunohistochemistry Analysis: A representative lot detected PEG in Immunohistochemistry applications (Wang, H., et. al. (2012). J Virol. 86(11):6286-302).Western Blotting Analysis: A representative lot detected PEG in Western Blotting applications (Ehrlich, G.K., et. al. (2009). J Mol Recognit. 22(2):99-103).ELISA Analysis: A representative lot detected PEG in ELISA applications (Hao WR et al (2018). Sci. Rep. 8(1); 4256; Chang, C.J., et. al. (2017). Nat Commun. 8(1); 522; Henry, C.E., et. al. (2016). Acta Biomater. 43:61-70).
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: All species
• Gene Alias: Polyethylene Glycol
• Isotype: IgM κ
• Purification Method: Purified by ion-exchange chromatography
• Test Specificity: Clone AGP4 specifically detects proteins conjugated with low to high molecular weight methoxy and amino polyethylene glycols (PEGs).

• Clone: AGP4
• Applications: ELISA, Immunohistochemistry, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Signaling
• Formulation: Purified mouse monoclonal antibody IgM in PBS with 0.04% sodium azide.
• Immunogen: Polyethylene glycol-conjugated to E.coli beta-glucuronidase.
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.