PF4 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P05BT25	Invitrogen™ 500P05BT25UG	25 μg
500P05BT50	Invitrogen™ 500P05BT50UG	50 μg
50-271-8720	Invitrogen™ 500P05BT500UG	500 μg

Description

AA Sequence of recombinant protein: EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human PF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PF-4 (500-P05) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P05BT-1MG will be provided as 2 x 500 μg

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).

Specifications

• Antigen: PF4
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P02776
• Gene ID (Entrez): 5196
• Immunogen: E.coli-derived, 7.8 kDa Recombinant Human PF-4 (CXCL4).
• Purification Method: Antigen affinity chromatography
• Gene: Pf4

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; Cxcl4; Endothelial cell growth inhibitor; Iroplact; oncostatin-A; PF4; PF-4; Pf4a; Platelet factor 4; Platelet factor 4, short form; RATPF4A; Scyb4
• Gene Symbols: Pf4
• Primary or Secondary: Primary
• Content And Storage: -20°C