Learn More
Thermo Scientific™ POROS™ CaptureSelect™ IgA Affinity Column, 4.6 x 50 mm
Description
Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentations broths, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle. POROS CaptureSelect IgA HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands. The combination allows three-minute quantitation of IgA.
- Broad utility—POROS A, G, and CaptureSelect affinity ligands address most biological drug classes
- Rapid—POROS resins offer fast quantitation or purification of immunoglobulins, fusion proteins
- Hands-off operation—HPLC method can be automated for streamlined operation
- Robust—allows for high-performance purification over hundreds of chromatography cycles
High Speed with High Specificity
The combination of CaptureSelect IgA affinity ligands and POROS 20 μm resin provides a high flow rate, high performance chromatography solution for the rapid titer determinations and small-scale purification of IgA.
POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and allows for an eluted product that is highly purified and suitable for further analysis, including mass spectrometry.
Automation
POROS affinity chromatography columns are suitable for use with HPLC systems, which allows for the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligands enable one method and buffer system to be used across a class of molecules, streamlining analytical approaches.
Multiple Cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regime can result in strong re-use performance. POROS affinity chromatography columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.
Specifications
Specifications
| Capacity (Metric) | 0.8 mL |
| Matrix | POROS |
| Content And Storage | Column includes EZ grip fittings and column test certificate. Store at 2°C to 8°C. DO NOT FREEZE. |
| Elution Volume Max | 29 column volumes |
| Elution Volume Min | 14 column volumes |
| For Use With (Equipment) | FPLC |
| High-throughput Compatibility | Automated Protocols |
| Max. Operating Pressure | 2600 psi (180 bar) |
| Particle Size | 20 μm |
| Product Line | CaptureSelect™, POROS™ |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
Your input is important to us. Please complete this form to provide feedback related to the content on this product.