Promega Alkaline Phosphatase, Calf Intestinal

Description

Alkaline Phosphatase catalyzes the hydrolysis of 5'-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.

• Catalyzes the Hydrolysis of 5′ Phosphate Groups from DNA, RNA, and r- and dNTPs
• Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μL
• Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications

Specifications

• Concentration: 1 U/μL
• Color: Colorless
• Components: 10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
• pH: 8
• For Use With (Application): Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
• Storage Buffer: 10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
• Content And Storage: -30°C to -10°C
• Source: Calf intestinal mucosa
• Quantity: 1000 U
• Product Type: Alkaline Phosphatase
• Form: Liquid