Promega GoTaq™ Hot Start Polymerase: 5X

Description

Includes

GoTaq Hot Start Polymerase is supplied with 5X Colorless GoTaq Flexi Buffer, 5X Green GoTaq Flexi Buffer, and 25mM MgCl₂.

GoTaq™ Hot Start Polymerase contains the high-performance GoTaq™ DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two minutes.

• Robust PCR with Convenient Room-Temperature Setup
• Minimize nonspecific amplification and primer-dimers
• Optimize reaction conditions with supplied Flexi Reaction Buffer and MgCl2 solution
• Direct-to-gel convenience
• Supplied at a concentration of 5u/μL

• U.S. Patent No. 6,242,235, Australian Patent No. 761757, Canadian Patent No. 2,335,153.
• Licensed under U.S. Patent Nos. 5,338,671 and 5,587,287 and corresponding patents in other countries.

Specifications

• Concentration: 5 U/μL
• Content And Storage: 5X Hot start Polymerase (1 x 100U), colorless flexi Buffer (1 x 1mL), 5X green flexi Buffer (1 x 1mL), MgCl2 (1 x 750μL). Store at −30 to −10°C
• Quantity: 100U
• For Use With (Application): Cloning
• Activation Time: 2 min.