Promega Restriction Enzymes - Mbo II (B)

Description

Enzymes that recognize short, specific (often palindromic) DNA sequences in which the enzymes then cleave.

• Incubate buffer B at 37°C
• Moraxella bovis ATCC 10900
• 10mM Tris-HCl (ph 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol
• Store at -20°C

Sensitive to overlapping dam methylation, MboII produces a single-base overhang, which is difficult to ligate

Specifications

• Concentration: 2 to 10 U/μL
• Components: 1 x 1mL 10X Buffer B, 1 x 150μL Bovine Serum Albumin, Acetylated, 1 x 100U MboII, 1 x 0.25mL Muti-Core™ 10X Buffer
• Incubator Temperature: 37°C
• pH: 7.3
• For Use With (Application): Enzymes that recognize short, specific DNA sequences in which the Enzymes then cleave
• Storage Buffer: 10mM Tris HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
• Quantity: 100 U
• Cut Site: GAAGA(N)8. CTTCT(N)7
• Product Type: MboII
• Form: Liquid