Promega T7 RNA Polymerase

Description

DNA-dependent RNA polymerase for in vitro transcription.

• DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences
• >90% pure as determined by SDS polyacrylamide gel electrophoresis
• Free of detectable levels of contaminating RNase and DNase activity (
• Incorporates 32P, 33P, 3H and 35S nucleoside triphosphates

Specifications

• Concentration: 10 to 20 U/μL
• Color: Colorless
• Components: 5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
• Purity: >90%
• pH: 7.7
• For Use With (Application): Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
• Storage Buffer: 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
• Content And Storage: -30°C to -10°C
• Source: Recombinant E. coli strain
• Quantity: 5000 U
• Product Type: T7 RNA Polymerase
• Inhibitors: Recombinant RNasin™ Ribonuclease
• Form: Liquid