Thermo Scientific™ Luminaris HiGreen qPCR Master Mix, fluorescein

Description

Includes

Luminaris HiGreen Fluorescein qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer. The master mix contains fluorescein passive reference dye for specific real-time platfo

Thermo Scientific Luminaris Color HiGreen and Luminaris HiGreen qPCR Master Mixes are universal ready-to-use solutions optimized for qPCR and two-step RT-qPCR.

• UDG in the master mix to prevent carry-over contamination
• Equal performance of blue and colorless master mixes
• Versions with premixed ROX or fluorescein for different real-time platforms
• Standard cycling protocols
• Excellent qPCR performance
• Specificity: Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity: single copy detection
• Wide linear range: accurate quantification across 9 orders of magnitude
• Reproducibility and convenience: ready-to-use 2X master mix

Specifications

• Concentration: 2X
• Content And Storage: Contains:
  • 2 x 1.25 mL Luminaris HiGreen Fluorescein qPCR Master Mix (2X) containing Hot Start Taq DNA Polymerase, UDG, dNTPs (with dUTP) and SYBR Green I in an optimized PCR buffer; sufficient for 250 x 20 μL reactions. The master mix contains fluorescein passive reference dye for specific real-time platforms.
  • 2 x 1.25 mL Nuclease-Free Water
  
  Store at -20°C.
• Detection Method: SYBR
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: Taq DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): BioRad iCycler iQ, BioRad MyiQ, BioRad iQ5
• Product Line: Luminaris
• Product Type: qPCR Master Mix
• Sufficient For: 250 Reactions
• For Use With (Application): Gene Expression
• Fidelity (vs. Taq): 2 X
• No. of Reactions: 250 Reactions
• Sample Type: DNA, dsDNA