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Molecular Probes™ Vybrant™ DyeCycle™ Orange Stain
Description
Vybrant™ DyeCycle™ Orange Stain is a cell permeable DNA dye that can be used for cell cycle analysis on 488 nm or 532 nm laser lines.
Precise—accurate cell cycle analysis in living cells
Safe—low cytotoxicity for cell sorting and additional live cell experiments
Simple, robust staining protocol
View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.
Precise
Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant™ DyeCycle™ Orange Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).
Low Cytotoxicity
Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant™ DyeCycle™ Orange Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.
Simple, Robust Staining Protocol
For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2μL of Vybrant™ DyeCycle™ Orange Stain. Final stain concentration is 10μM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on flow cytometer using 488 nm excitation and orange emission.
View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.
Precise
Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant™ DyeCycle™ Orange Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).
Low Cytotoxicity
Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant™ DyeCycle™ Orange Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.
Simple, Robust Staining Protocol
For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2μL of Vybrant™ DyeCycle™ Orange Stain. Final stain concentration is 10μM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on flow cytometer using 488 nm excitation and orange emission.
Specifications
Specifications
| Concentration | 5 mM |
| Content And Storage | Contains 1 vial of Vybrant™ DyeCycle™ orange stain (5 mM in DMSO). Store at ≤-20°C, desiccate and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Equipment) | Flow Cytometer |
| Product Type | Stain |
| Dye Type | Vybrant™ DyeCycle™ Orange |
| Emission | 532/488 |
| Form | Solution |
| Format | Tube(s) |
| Product Line | DyeCycle |
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For Research Use Only. Not for use in diagnostic procedures.
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