Stain for detection and location of proteins. Protocol: Wash blot in 0.2% Tween/PBS, with three changes of 15 min. each at RT. Place blot in stain solution for 5 min. at RT with agitation. Destain in 90% methanol/2% acetic acid/8% water.Stain for proteins and total carbohydrates in plants.A histological, protein, and nucleic acid dye used in electrophoresisHigh Purity Grade EM (615nm, H2O) > 28,500Synonym: Acid Black 1; Amido Black 10B; Buffalo Black NBRRefractive index: n20D 1.77 (Predicted)HS Code : 3204.12IUPAC_Name: disodium (6Z)-4-amino-3-(4-nitrophenyl)diazenyl-5-oxo-6-(phenylhydrazinylidene)naphthalene-2,7-disulfonateRTECS Number: QJ6196000