Glycosyl compounds
![Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 100 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71800-0.1.JPG-250.jpg)
Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 100 Milligrams
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
eMolecules Combi-Blocks Sodium ascorbate 1kg 290696287 QE-4378 97 000 134-03-2 MFCD00082340 198 106 C6H7NaO6
Combi-Blocks Sodium ascorbate 1kg 290696287 QE-4378 97 000 134-03-2 MFCD00082340 198 106 C6H7NaO6
Sigma Aldrich Fine Chemicals Biosciences Poly D lysne hydrobromde500MG
Poly D lysne hydrobromde500MG
Medchemexpress LLC D-Galacto-D-mannan (carob galactomannan) | 11078-30-1 | MFCD00146683 | 95.0% | Not specified (polymeric; variable molecular weight) | C18H32O16 | 100 MG
D-Galacto-D-mannan is a carob-derived (Ceratonia siliqua) linear, nonionic polysaccharide used in biochemical applications as a thickener, stabilizer, co-gellant, and fat replacer. The material is supplied as a white powder for research use only and is typically stored refrigerated or frozen per handling guidance.
Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl alpha-D-glucopyranoside >=99% | 3767-28-0 | MFCD00064088 | 25G
4-Nitrophenyl alpha-D-glucopyranoside >=99% | Purity: >=99% | Mol Wt: 301.25 | 3767-28-0 | MFCD00064088 | 25G
Sigma Aldrich Fine Chemicals Biosciences 4-Methylumbelliferyl a-D-galactopyranoside | 38597-12-5 | MFCD00063278 | 250mg
4-Methylumbelliferyl a-D-galactopyranoside | Purity: ≥98% | Mol Wt: 338.31 | 38597-12-5 | MFCD00063278 | 250mg
Sigma Aldrich Fine Chemicals Biosciences D-(+)-Cellobiose suitable for microbiology, >=99.0% | 528-50-7 | MFCD00136034 | 250G
D-(+)-Cellobiose suitable for microbiology, >=99.0% | Purity: >=99.0% (HPLC); >=99.0% | Mol Wt: 342.3 | 528-50-7 | MFCD00136034 | 250G
Sigma Aldrich Fine Chemicals Biosciences Amylose from potato used as amylase substrate | 9005-82-7 | MFCD00134652 | 5G
Amylose from potato used as amylase substrate | 9005-82-7 | MFCD00134652 | 5G
Sigma Aldrich Fine Chemicals Biosciences Methyl alpha-D-mannopyranoside >=99.0% (HPLC) | 617-04-9 | MFCD00063262 | 100G
Methyl alpha-D-mannopyranoside >=99.0% (HPLC) | Purity: >=99.0% (HPLC) | Mol Wt: 194.18 | 617-04-9 | MFCD00063262 | 100G