Sphingolipids
Sigma Aldrich Fine Chemicals Biosciences Colorimetric Sphingomyelin
Sphingomyelinase (SMase) or sphingomyelin phosphodiesterase catalyzes the hydrolytic cleavage of sphingolipid to phosphocholine and ceramide a second messenger involved in multiple cellular responses including the cellular response to stress. There are at least 5 types of SMases identified which are classified according to their cation dependence cellular location and pH optima for activity and include the acidic and neutral sphingomyelinase. Neutral SMases along with the acidic SMases are thought to be major contributors to the production of ceramide in response to cellular stress.
Medchemexpress LLC Lysophosphatidylcholine (15:0) | 108273-89-8 | MFCD00674356 | 99.6% | 481.60 | C23H48NO7P | 5 MG
15:0 Lyso PC is a lysophosphatidylcholine used as an analytical lipid standard and research reagent for studies of membrane remodeling, inflammatory signaling, and lipid biomarkers in cardiovascular disease. It is supplied as a solid with high purity suitable for analytical and biochemical applications.
- High purity (99.6%) for reproducible analytical results.
- Molecular weight 481.60 and formula C23H48NO7P for precise identification.
- White to off-white solid appearance for easy handling.
- Available in small mg-scale amounts suitable for research use.
- Stable as a powder at -20°C for long-term storage.
Cayman Chemical C16 Ceramide d18 1 16 0
A sphingolipid; levels are increased by PPMP or MAPP in isolated human neutrophils and are correlated with an increase in apoptosis; accumulates in the lungs of patients with cystic fibrosis compared with the lungs of patients with pulmonary hypertension; plasma levels are increased in patients with acute coronary syndrome, and an increased plasma ratio of C16 to C24:0 ceramide is associated with major adverse cardiac events; levels are increased in tumor tissue from patients with breast cancer and is associated with metastatic cancer
Cayman Chemical Sphinganine d18 0
A precursor of ceramide and sphingosine; can emulate S1P in cell signaling or have opposite intracellular effects
Cayman Chemical C12 NBD Ceramide d18 1 12 0
C12 NBD ceramide (d18:1/12:0) is a long-chain fluorescent ceramide analog that can be used for the measurement of alkaline and neutral ceramidase activity.{14063} It is hydrolyzed efficiently by alkaline ceramidase from P. aeruginosa and by neutral ceramidase from mouse liver. In addition, alkaline and neutral ceramidases from a variety of mammalian cell lines also hydrolyze C12 NBD ceramide (d18:1/12:0). Acid ceramidase does not appear to utilize C12 NBD ceramide (d18:1/12:0) as a substrate. The possibility exists forC12 NBD ceramide (d18:1/12:0) to also be applied to the measurement of ceramide kinase and/or sphingomyelin synthase activity.
Apexbio Technology LLC Lyso-Tracker Green(Synonyms: LysoTracker Green DND-26, LysoTracker Green, LysoTracker Green lysosomal probe), 50ul.
Lyso-Tracker Green is a fluorescent small-molecule probe designed specifically for labeling lysosomes in living cells. As a weakly basic fluorophore, it accumulates selectively in acidic cellular compartments, primarily lysosomes, due to protonation under low pH conditions. Unlike conventional dyes such as acridine orange or neutral red, Lyso-Tracker Green exhibits superior organellar selectivity and requires only nanomolar concentrations to produce robust fluorescent labeling. It is widely utilized in biomedical research for imaging lysosomal localization and analyzing cellular physiology in live-cell assays, but is not compatible with fixed-cell staining protocols.