Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Promocell GmbH PromoCell Hepes BSS (30 mM) 30 ml, HEPES Buffered Saline Solution
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.HEPES Buffered Saline Solution (30 mM).
Seracare Life Sciences Inc 10X TRIS-GLYCINE TRANS BUFFER
10X Tris-glycine Transfer Buffer is intended for Western blotting and gel electrophoresis. It is ideal for preparing a standard Western blot transfer buffer (Towbin) and for use as a gel electrophoresis buffer for native Tris-glycine gel without SDS. pH=8.3. Conductivity controlled.
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.4) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Pyridoxal which is an aldehyde has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.
Sigma Aldrich Fine Chemicals Biosciences ADA 98 titration1KG
ADA is a zwitterionic buffer used in biochemistry and molecular biology. It is one of the Good buffers developed in the 1960 to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of ADA and its physical properties. The useful range of ADA buffer in aqueous SOLUTIONon is 6.0 - 7.2.
Growcells PBS 10X 2L EA
Sterile 10X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 80g, KCl 2g, Na2HPO4 14.4g, KH2PO4 2.4g
Growcells TAE BUFFER 10X 2L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 48.46g TRIS Base, 11.4ml Acetic Acid, 3.72g EDTA. pH 8.3
Research Products International Corp HEPES Buffered Saline Solution 2X, 500 Milliliters
2X Formulation:
KCI: 10 mM
Na2HPO4: 1.5 mM
Dextrose: 12 mM
HEPES: 50 mM
pH: 7.0
Electron Microscopy Sciences Hepes Buffer Solution 0.2M, 7.0-8.0 pH 500 ML
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged. Desired pH should be specified when ordering.
pH: 7.0-8.0
FUJIFILM BIOSCIENCES INC Iscoves Modified Dulbeccos Med
Iscoves Modified Dulbeccos Medium IMDM is a modification of Dulbeccos Basal Medium Eagle BME that uses high glucose and pyruvate additional amino acids 25mM HEPES selenium and other components IMDM is designed for serumfree growth of hemopoietic cells in culture and supports the growth ofLymphocytes and a variety of other hybrid cells
Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 1 Kg GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade
Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade .