Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova Tris Glycerol Buffer pH 8.0. 1000mL, Endotoxin Tested, Sterile 2 pack.
20mM Tris,25mM Sodium Chloride,2.5% Glycerol
Teknova 0.45% Saline Solution, Sterile. 500mL 4 pack.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Teknova CTAB Extraction Solution. 250mL. 4 Pack.
2.0% CTAB, 100mM Tris-HCl, pH 8.0,20mM EDTA, pH 8.0, 1.4M Sodium Chloride
Teknova 10X TBS, pH 7.4. 1000mL, Sterile (247mM Tris, 27mM KCl, 1.37M NaCl) 2 pack.
247mM Tris-HCl,27mM Potassium Chloride,1.37M Sodium Chloride
Teknova 1M MOPS, pH 7.0. 1000mL, Sterile. Endotoxin Tested. 2 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
Teknova 5X TBE DNA Sequencing Grade Solution. 4L, Non-Sterile. 4 pack.
445mM Tris, 445mM Boric Acid, 10mM Ethylenediaminetetraacetic acid (EDTA)
Teknova 10X TE Buffer Solution. 250mL, DNase/RNase, PCR Grade, Sterile. 4 pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Teknova 20X TBS with 0.1% Tween-20. (400mM Tris, 3M NaCl) 1000mL, Sterile. 2 pack.
3M Sodium Chloride, 400mM Tris-HCl
Teknova 1X TBS, Modified. 1000mL, Sterile. (150mM NaCl, 50mM Tris) 2 pack.
50mM Tris-HCl, 150mM Sodium Chloride
MSC Value Collection Magnifier, 20x, Dual Lens, Round, Metal
The Value Collection magnifier is a durable tool designed for detailed inspection. It features a high 20x magnification, a dual-lens system, and a robust metal construction, making it suitable for various precision tasks.
- 20x maximum magnification
- Dual lens system
- Round lens shape
- Metal construction
Gold Biotechnology Inc MES Free Acid 250 g
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered, 500mL, 10/Case
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
Research Products International Corp Potassium Phosphate, Dibasic, 10 KG
Potassium Phosphate Dibasic, Anhydrous (dipotassium phosphate) is a buffering agent commonly used in molecular biology, biochemistry, and cell culture. It effectively maintains pH in the neutral to slightly alkaline range (7.0–8.0), making it ideal for applications such as protein purification, enzyme assays, and cell culture media. Its strong buffering capacity ensures experimental consistency. In addition to pH control, it serves as a potassium ion source—supporting cellular processes and metabolism. Its dual role as a pH stabilizer and nutrient makes it valuable in media formulation and biochemical research.
Research Products International Corp TG 10X Solution, 2 L
10X Formulation:
Glycine: 1.92 M
pH: 8.5
Add methanol according to protocol