Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc RNA Sample Loading Buffer (No Eth Bromide), 1 ml
RNA Sample Loading Buffer (No Eth Bromide), 1 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml.
![eMolecules 1420955-72-1 | tert-butyl 8-(hydroxymethyl)-2-azaspiro[4.5]decane-2-carboxylate | MFCD24384294 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502159466.JPG-250.jpg)
eMolecules 1420955-72-1 | tert-butyl 8-(hydroxymethyl)-2-azaspiro[4.5]decane-2-carboxylate | MFCD24384294 | 1g
Medchem Express | ZLN024 (hydrochloride) | 5mg | 446267722 | HY-16708A | 1883548-91-1 | MFCD28166492 | 361.680 | C13H14BrClN2OS
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CHEMSCIENCE Soluble Chloride for Cl
• This product is supplied in five lot of 500 g each of known concentration ( % concentration availiable upon request.)
• Detailed purity information, along with the Safety Data Sheet (SDS) and Technical Data Sheet (TDS), will be provided upon purchase.
eMolecules 5704-04-1 | Tricine | Oakwood Chemical | MFCD00004277 | 179.172 | C6H13NO5 | 98.000 | OCC(CO)(CO)NCC(O)=O | 1g | 537694332
Tricine | Oakwood Chemical | 5704-04-1 | MFCD00004277 | 179.172 | C6H13NO5 | 98.000 | OCC(CO)(CO)NCC(O)=O | 1g | 537694332
Alkali Scientific TRIS CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
TRIS CAPS Buffer 10X Solution is designed for use in capillary electrophoresis and other advanced electrophoretic techniques. This formulation, available in two 500mL bottles, ensures convenient preparation and consistent results during capillary separation of charged biomolecules. The 10X solution can be diluted to create the working concentration needed for various electrophoresis applications. TRIS CAPS buffer is particularly useful in separating proteins and nucleic acids in a high-resolution format. This buffer is often used in protein and DNA/RNA electrophoresis protocols, providing stable pH conditions that enhance separation and improve sensitivity during detection.
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661163.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 1 L
TBE 10X Solution is a buffered solution commonly used in molecular biology and biochemistry. This 1-liter bottle contains a highly concentrated solution of Tris, borate, and EDTA (ethylene diamine tetraacetic acid), which is used as a running buffer for agarose gel electrophoresis. TBE helps maintain the pH and ionic strength of the electrophoresis buffer system, which is critical for the proper migration of nucleic acids. This 10X concentrated solution can be diluted to 1X for use in standard gel electrophoresis procedures. It is an essential reagent for DNA analysis, particularly in applications involving high-resolution separation of nucleic acid fragments.
Bioworld Bis-Tris Propane, 1 kg
A non-zwitterionic buffer that is useful in the pH 6.3-9.5 rangeSynonym: 1,3-Bis[tris(hydroxymethyl)methylamino]propane Appearance: SolidBoiling Point : 573.3 °CDensity : 1.326 g/cm3IUPAC_Name: 2,2'-(1,3-Propanediyldiimino)Bis[2-(Hydroxymethyl)-1,3-Propanediol]Melting Point: 164-165 °CPH Value: 6.3 - 9.5