Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt >=99% (titration) | 76836-02-7 | MFCD00064349 | 1KG
PIPES disodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 1KG
Research Products International Corp TAPS, 5 KG
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
Teknova 1M HEPES, pH 7.5, 20L Bag, Sterile. 4 Pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Sigma Aldrich Fine Chemicals Biosciences MOPS hemisodium salt >=99% (titration) | 117961-20-3 | 250G
MOPS hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 220.25 | 117961-20-3 | 250G
Alkali Scientific MOPSO Sodium Salt, 500 G
MOPSO Sodium Salt, 500 G, is a high-quality buffering reagent used to stabilize pH in laboratory experiments, particularly those involving proteins, nucleic acids, and enzyme reactions. MOPSO is known for its ability to maintain a stable pH in the range of 6.5 to 7.9, which is essential for many biochemical assays and protocols. This larger 500 g package is ideal for high-volume or repeated experiments and provides a cost-effective solution for laboratories working on large-scale studies or industrial applications. MOPSO is widely used in cell culture, protein analysis, and other critical research areas requiring reliable pH control.
Bioworld ACES, 100 g
ACES, High Purity Grade N-[carbamoylmethyl]-2-Aminoethane-sulfonic acidpKa: (20°C): 6.8+0.1Appearance: White crystalsHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.1-7.5
Teknova 20 x MOPS/SDS Running Buffer. 500mL, Sterile. 8 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
ILEX LIFE SCIENCES LLC PAN-Biotech HEPES Buffer 1M (100 ml)
HEPES Buffer 1M. QUANTITY: 100 ml. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: 2 to 8 degrees Celsius. DESCRIPTION: HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. COMPOSITION: HEPES 238.31 g/L.
![Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660773.jpg-250.jpg)
Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 100 G
BES (N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid) is a biochemical reagent commonly used as a buffering agent in laboratory research. The 100g quantity provides a stable and reliable source of BES for a wide range of applications, including protein studies, enzyme assays, and electrophoresis. BES is valued for its ability to maintain a stable pH environment in biochemical systems, especially when working with proteins and other biomolecules sensitive to pH variations. It is commonly used in preparation of buffers for biological assays and for maintaining a consistent pH during biochemical reactions. This reagent is indispensable in labs focused on molecular biology, biochemistry, and clinical diagnostics.
![Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661288.jpg-250.jpg)
Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 1 Kg
TAPS, or Tris(hydroxymethyl) aminopropanesulfonic acid, is a high-purity buffering agent provided in the free acid form for use in laboratory settings. This 1kg quantity is perfect for large-scale buffer preparation, particularly in molecular biology, electrophoresis, and protein studies. TAPS helps maintain a stable pH in solutions, making it ideal for a variety of biological assays, including enzyme reactions and chromatography. The high purity of this reagent ensures that it will not interfere with sensitive experiments, delivering consistent and reproducible results. The 1kg size offers cost-effective, long-term use for labs that regularly prepare TAPS-based buffers.
Chem-Impex International, Inc. 3-Cyclohexylamino-1-propanesulfonic acid | 1135-40-6 | MFCD00003837 | 250G
3-Cyclohexylamino-1-propanesulfonic acid, 1135-40-6, MFCD00003837, 250G
Biotang Inc HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545
HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545.
Medchemexpress LLC Ada disodium | 41689-31-0 | 98.0% | 5 G
ADA (disodium) is a buffer salt derived from adenosine deaminase, commonly employed in biochemical research, particularly for preparing enzyme buffers.
- Used in biochemical research.
- Specifically used in the preparation of enzyme buffers.