Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
MOLTOX Molecular Toxicology Inc 20 mM Tris-HCL, pH 8.6, 1000 mL/bottle, Store at Room Temperature
MOLTOX manufactures a wide variety of buffers for your laboratory needs. All MOLTOX reagents are accompanied by a GLP-compliant Formulation and Quality Control statement.
MOLTOX Molecular Toxicology Inc Saline Solution 0.85% Nacl, 9 mL/tube, 10 tubes/box, Store at Room Temperature
MOLTOX offers a wide array of solutions and buffers for a variety of laboratory research needs. All reagents are accompanied by a GLP-compliant Formulation and Quality Control statement.
Biotang Inc HEPES Lysis Buffer with Triton (2X), 125 ml
HEPES Lysis Buffer with Triton (2X), 125 ml.
Abcam Phosphate Buffered Saline (PBS) Casein ELISA Reagent, 100ML
Phosphate Buffered Saline (PBS) Casein ELISA Reagent is a buffer designed for ELISA assay development.
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences BICINE BioXtra 99 titratio1KG
BICINE BioXtra 99 titratio1KG
![Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75070-100.0.JPG-250.jpg)
Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 100 Grams
HEPPSO (N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)) is a zwitterionic buffering agent commonly used particularly in biochemical and molecular biology research. It is valued for its ability to maintain a stable pH in a variety of biological assays and experiments.
HEPPSO is often preferred for its buffering capacity over a wide pH range, typically between pH 6.8 to 8.2, making it suitable for various applications such as protein purification, enzyme assays, cell culture, and electrophoresis.
Additionally, HEPPSO is less likely to interfere with biological processes compared to some other buffering agents, making it a versatile choice for maintaining optimal conditions in biological experiments.