Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid BioUltra, >=99.0% (KT) | Purity: >=99.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 1KG
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
Research Products International Corp Bicine Ultra-Pure, 100 Grams
Zwitterionic buffer used in a variety of molecular biology applications such as protein resolution, protein crystallization and SDS-PAGE.
pKa @ 25°C: 8.3
pH Range: 7.6-9.0
Melting Range: 190-192°C
Research Products International Corp TRIS ACETATE ULTRA PURE, 100 GRAM
Moisture: <0.3%
pH (0.1M, H2O) @25°C: 6.0-7.0%
Heavy Metals: 0.001%
Chem-Impex International, Inc. MOPS sodium salt | 71119-22-7 | MFCD00064350 | 1KG
MOPS sodium salt, 71119-22-7, MFCD00064350, 1KG
Sigma Aldrich Fine Chemicals Biosciences Piperazine | 110-85-0 | MFCD00005953 | 100 g
Piperazine | Purity: ≥99.0% | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 100 g
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
PHOSPHATE Phosphate buffered saline
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 250g
CAPS | Purity: 99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 250g
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic Acid | 60-00-4 | MFCD00003541 | 100g
Ethylenediaminetetraacetic Acid | Purity: 98% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 100g
Sigma Aldrich Fine Chemicals Biosciences MOPS hemisodium salt >=99% (titration) | 117961-20-3 | 100G
MOPS hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 220.25 | 117961-20-3 | 100G
Sigma Aldrich Fine Chemicals Biosciences CHES | 103-47-9 | MFCD00003835 | 1kg
CHES | Purity: 99% | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 1kg
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 1kg
CAPS | Purity: 99% | MW: 221.32 | 1135-40-6 | MFCD00003837 | 1kg
Sigma Aldrich Fine Chemicals Biosciences MES hydrate 99.5 titration25KG
MES is one of the Good buffers the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility making it impermeable to membranes.
Research Products International Corp PBS 200ml Tablets 500 Tab
PBS, 200ml Tablets refer to pre-weighed tablets used to prepare a specific volume of PBS (Phosphate Buffered Saline) solution.
The buffer tablets are commonly used buffer solution in biological and biochemical research. PBS serves as a versatile buffer for various applications, including cell culture, immunohistochemistry, protein and nucleic acid research, and diagnostic assays. It helps maintain a stable pH and osmolarity, providing optimal conditions for biological reactions and preserving the integrity of cells and biomolecules.
The PBS, 200ml Tablets are designed to simplify the preparation process of PBS solutions. Each tablet contains pre-determined amounts of sodium chloride, sodium phosphate dibasic, and potassium phosphate monobasic. By using these tablets, researchers can save time and ensure accuracy by eliminating the need to weigh and measure individual components.