Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate (Standard) | 145224-94-8 | 99.95% | 50 MG
MES monohydrate (Standard) is the analytical standard of MES (monohydrate), intended for research and analytical applications. MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5-7.0. As a Good's buffer, it is widely used in biochemistry and molecular biology experiments.
- Used for research and analytical applications.
- Amphoteric ion buffer.
- Buffer capacity ranging pH 5.5-7.0.
- Widely used in biochemistry and molecular biology experiments.
- Suitable for cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Commonly used in qualitative, quantitative, and methodological research experiments in HPLC, GC, and MS.
Medchemexpress LLC Bicine | 150-25-4 | 98.0% | 10 G
Bicine is a buffer utilized in physiological research. It is a derivative of the simple amino acid glycine that enhances the sensitivity of guanosine enzyme histochemical detection. This product appears as a white to off-white solid.
- Buffer for physiological research
- Derivative of amino acid glycine
- Enhances sensitivity of guanosine enzyme histochemical detection
- White to off-white solid appearance
- Molecular weight: 163.17
- Chemical formula: C6H13NO4
- Shipped at room temperature
- Stable at room temperature for 3 years
- Stable in solvent at -80°C for 2 years or -20°C for 1 year
- For research use only
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
Sigma Aldrich Fine Chemicals Biosciences BICINE BioXtra 99 titratio1KG
BICINE BioXtra 99 titratio1KG
LICORbio Odyssey MES SDS Running Buffer Powder (pack of 5)
Odyssey Mini Gels are polyacrylamide gels designed to separate a wide range of protein sizes via PAGE or SDS-PAGE. Each box contains 10 pre-cast gels. Our gels feature larger well volumes for easier sample loading and are cast in a neutral pH buffer to increase gel stability. They also run at neutral pH, which minimizes protein modification compared to Tris-glycine gels. Odyssey Mini Gels are available in several gradients with 10, 12, or 15 numbered wells. Our gels feature a shelf life of up to 6 months at room temperature and 12 months at 2–8C.
Quality Biological Inc HEPES Buffer (1 M), pH 7.3, 100 mL
HEPES is a zwitterionic organic chemical buffering agent. HEPES (1M) is used as a buffer alternative from sodium bicarbonate for cell culture and is widely used in many biochemical reactions and as a buffering agent in some cell culture media. HEPES is reportedly superior to NaHCO3 in controlling pH in tissue and organ culture.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Performance Tested
pH: 7.2-7.4
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Research Products International Corp TES, Sodium Salt, 25 G
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
eMolecules 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 20656-08-0 | MFCD00141691 | 1g
Matrix Scientific | 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 1g | 389721058 | 45569 | 95.000 | 20656-08-0 | MFCD00141691 | 234.080 | C9H9Cl2NO2
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![Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661552.jpg-250.jpg)
Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 250 G
PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt) is a high-purity reagent provided in a 250G pack. It is commonly used as a buffering agent in biochemical and laboratory applications due to its stability at a wide range of pH levels. PIPES is primarily used in the preparation of buffers for biological assays and research, as it does not interfere with biological systems. The sesquisodium salt form helps maintain the pH in both acidic and basic environments. This reagent is suitable for cell culture, enzyme assays, and other biochemical studies where precise pH control is essential. It is also utilized in electrophoresis, chromatography, and immunological testing.
Apexbio Technology LLC HEPES(Synonyms: HEPES buffer, 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid), 250g, CAS: 7365-45-9.
HEPES (CAS 7365-45-9) is a zwitterionic sulfonic acid buffer commonly utilized in biomedical research due to its capacity to maintain physiological pH stability within a buffering range of approximately pH 6 8 to 8 2 Mechanistically HEPES modulates intracellular pH balance thereby promoting lysosomal biogenesis and supporting associated cellular processes It is routinely employed in cell culture systems to precisely regulate environmental conditions and is relevant in biochemical assays involving specific cellular signaling pathways and intracellular pH-dependent phenomena
Sigma Aldrich Fine Chemicals Biosciences HEPES Sodium Salt | 75277-39-3 | MFCD00036463 | 100g
HEPES Sodium Salt | Purity: 99% | Mol Wt: 260.29 | 75277-39-3 | MFCD00036463 | 100g
Boston Bioproducts Inc PIPES Buffer (0.5 M, pH 7.0) - 100ml
The PIPES Buffer is a zwitterionic buffering agent commonly used in biochemistry and molecular biology for its ability to maintain a stable pH. With a pKa of approximately 6.8, PIPES is effective in the pH range of 6.1 to 7.5, making it suitable for cell culture, enzyme assays, protein purification, and as a running buffer in gel electrophoresis. PIPES is also used in electron microscopy, protein crystallization, and isoelectric focusing and chromatography.
Sigma Aldrich Fine Chemicals Biosciences HEPES BioXtra pH 5.0-6.5 150G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences MOPSO sodium salt 99 titra500G
MOPSO sodium salt 99 titra500G
Medchemexpress LLC 3-(N-morpholino)propanesulfonic acid sodium salt | 71119-22-7 | 99.8% | 100 G
MOPS sodium salt acts as a buffering agent within biological contexts. This buffer is critical for sustaining the appropriate pH levels in mammalian cell culture media. It also significantly enhances the thermal stability of bovine serum albumin.
- Functions as a buffering agent.
- Maintains pH of mammalian cell culture media.
- Improves thermal stability of bovine serum albumin.